Figure 4. (a) Venn diagram displaying differentially expressed proteinsafter exposure to TNF in different samples: cells, MVs and EXOs. For detailedlist see Supplementary Table 2. (b) Comparison of the fold-change (FC) ofproteins found to be significantly upor downregulated after exposure to TNF (p< 0.01, |FC|> 1.5) in both cells and EVs. The significant IPA canonicalpathways enriched in cell (c), MV (d) and EXO (e) samples. The significance(–log10 (p-value)) for each pathway are displayed. (f) Heat map showing the results of the IPA upstream regulator analysis.